Hydrocarbon and Lipid Microbiology Protocols: Genetic, Genomic and System Analys

Description: Hydrocarbon and Lipid Microbiology Protocols by Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales This Volume presents protocols for investigating the genetic, metabolic and ecological potential and functional analysis of microbial communities. FORMAT Hardcover LANGUAGE English CONDITION Brand New Publisher Description This Volume presents protocols for investigating the genetic, metabolic and ecological potential and functional analysis of microbial communities. Methods are described for the creation and bioinformatic assessment of metagenomic and metatranscriptomic libraries, and for metaproteomic analyses, which provide important insights into the metabolic potential and interactions of community members. These in turn lead to specific hypotheses concerning the functional contributions of individual populations in the community, which may be investigated by the stable isotope probing approaches described in this Volume, making it possible to identify those community members primarily responsible for particular functions. Methods for the direct extraction of proteins from environmental samples for sequencing and activity tests are presented, providing a broad overview of prevailing metabolic activities and of the types of microbe involved in them. Protocols for the analysis of nutrient flow through microbial communities and for the modelling of dynamic physiological interactions in communities are also provided. Lastly, the book presents a protocol for the quantitative assessment of permissiveness for the transfer of conjugative plasmids, important agents of physiological change and evolution in microbial communities.Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes. Back Cover This Volume presents protocols for investigating the genetic, metabolic and ecological potential and functional analysis of microbial communities. Methods are described for the creation and bioinformatic assessment of metagenomic and metatranscriptomic libraries, and for metaproteomic analyses, which provide important insights into the metabolic potential and interactions of community members. These in turn lead to specific hypotheses concerning the functional contributions of individual populations in the community, which may be investigated by the stable isotope probing approaches described in this Volume, making it possible to identify those community members primarily responsible for particular functions. Methods for the direct extraction of proteins from environmental samples for sequencing and activity tests are presented, providing a broad overview of prevailing metabolic activities and of the types of microbe involved in them. Protocols for the analysis of nutrient flow through microbial communities and for the modelling of dynamic physiological interactions in communities are also provided. Lastly, the book presents a protocol for the quantitative assessment of permissiveness for the transfer of conjugative plasmids, important agents of physiological change and evolution in microbial communities. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes. Table of Contents Introduction to genetic, genomic and system analyses for communities.- Genomic analysis of pure cultures and communities.- Protocols for metagenomic library generation and analysis in petroleum hydrocarbon microbe systems.- Preparation and analysis of metatranscriptomic libraries in petroleum hydrocarbon microbe systems.- Prokaryotic metatranscriptomics.- Defining a pipeline for metaproteomic analyses.- Generating enriched metagenomes from active microorganisms with DNA stable isotope probing.- DNA and RNA-based stable isotope probing of hydrocarbon degraders.- Protein stable isotope probing (Protein-SIP).- Protein extraction from contaminated soils and sediments.- Analysis of the regulation of the rate of hydrocarbon and nutrient flow through microbial communities.- Physiological modelling of complex bacterial communities.- Protocol for evaluating the permissiveness of bacterial communities towards conjugal plasmids by quantification and isolation of transconjugants. Feature Offers readily-reproducible step-by-step laboratory methods Provides helpful tips and tricks supporting the protocols Includes troubleshooting advice Contains practical laboratory guidance to promote successful results Details ISBN3662504499 Short Title HYDROCARBON & LIPID MICROBIOLO Series Springer Protocols Handbooks Language English ISBN-10 3662504499 ISBN-13 9783662504499 Media Book Format Hardcover DEWEY 660.62 Edition 1st Imprint Springer-Verlag Berlin and Heidelberg GmbH & Co. K Subtitle Genetic, Genomic and System Analyses of Communities Place of Publication Berlin Country of Publication Germany Edited by Balbina Nogales Pages 288 Year 2017 Publication Date 2017-01-06 Illustrations 30 Illustrations, color; 20 Illustrations, black and white; X, 288 p. 50 illus., 30 illus. in color. DOI 10.1007/978-3-662-50450-5 Author Balbina Nogales Birth 1946 Affiliation Universitat Heidelberg Position EDFRTR Qualifications Sir Publisher Springer-Verlag Berlin and Heidelberg GmbH & Co. KG Edition Description 1st ed. 2017 Alternative 9783662570623 Audience Professional & Vocational We've got this At The Nile, if you're looking for it, we've got it. With fast shipping, low prices, friendly service and well over a million items - you're bound to find what you want, at a price you'll love! TheNile_Item_ID:131036488;

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